Journal: Nature Communications

Article Title: Anchoring cortical granules in the cortex ensures trafficking to the plasma membrane for post-fertilization exocytosis

doi: 10.1038/s41467-019-10171-7

Figure Lengend Snippet: Impaired trafficking leads to delayed exocytosis and polyspermy. a Ovulated eggs from wild-type (left) and Mater Null (right) female mice were inseminated, cultured to two-cell embryos and stained with WGA-Alexa Fluor 633 lectin (red, zona pellucida) and Hoechst 33342 (blue, DNA). Scale bar, 20 μm. b The number of sperm in the perivitelline space (PVS) for each genotype (9–36 two-cell embryos from three biologically independent samples). Box plot includes the mean (horizontal line) and data between the 25th and 75th percentile. Error bars indicate the 90th and 10th percentiles; outliers are indicated by dots. *** p < 0.001 by two-tailed Student’s t -test. c In vivo fertilized Mater Het and Mater Null zygotes were fixed for staining with WGA-Alexa Fluor 633 (zona pellucida), phalloidin (F-actin) and DAPI (DNA). Arrows, supernumerary sperm in the PVS; pronuclei enclosed by dashed line. Scale bar, 20 μm. d Quantification of sperm in the PVS from c was determined for each genotype (13–60 embryos from three biological samples). *** p < 0.001 by chi-squared test. e Quantification of pronuclei in embryos from c . ** p < 0.01 by chi-squared test. f Ovulated eggs from wildtype (upper) and MyoIIA mNull (lower) were immunostained with WGA-Alexa Fluor 633 (magenta) and anti-MyoIIA (green). Representative confocal cross sections of z-projections are shown with a magnified view (inset) of the myoIIA region indicated by arrows. Scale bar, 20 μm. g After in vitro insemination, wild-type and MyoIIA mNull eggs were cultured to the 4-cell stage and fixed for staining with WGA-Alexa Fluor 633 (zona pellucida), phalloidin (F-actin) and DAPI (DNA). Arrows, supernumerary sperm in the perivitelline space; dashed lines, nuclei. Scale bar, 20 μm. h The number of sperm in the PVS for each genotype in g (15–18 embryos from two biologically independent samples). *** p < 0.001 by chi-squared test. Source data are provided as a Source Data file

Article Snippet: CGs were immunostained with the conjugated lectin LCA-FITC (Cosmo Bio USA) at a 1:100 dilution.

Techniques: Cell Culture, Staining, Two Tailed Test, In Vivo, In Vitro

Journal: PLoS ONE

Article Title: Changes in surface glycosylation and glycocalyx shedding in Trichobilharzia regenti (Schistosomatidae) during the transformation of cercaria to schistosomulum

doi: 10.1371/journal.pone.0173217

Figure Lengend Snippet: Lectins, saccharide inhibitors, and binding preferences of lectins.

Article Snippet: Glycocalyx shedding was induced by 7 lectins (UEA-I, LTA, Con A, LCA, SBA, JAC and MBL) at a concentration of 20 μg/ml ( ).

Techniques: Binding Assay